![[KO Validated] DNA Polymerase beta Rabbit pAb (A1681)](/upload/resize_cache/iblock/f90/340_340_140cd750bba9870f18aada2478b24840a/fiprgbo2dtn4r72blgyi3gr7gofl6imt.jpg "[KO Validated] DNA Polymerase beta Rabbit pAb (A1681)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] DNA Polymerase beta Rabbit pAb (A1681)
- Описание
- Характеристики
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Overview
Product name [KO Validated] DNA Polymerase beta Rabbit pAb Catalog No. A1681 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human DNA Polymerase beta (NP_002681.1). Sequence MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE Gene ID Swiss Prot Synonyms POLB Calculated MW 38kDa Observed MW 37kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:200 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:20 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples Jurkat, Mouse skeletal muscle Cellular location Cytoplasm, Nucleus Customer validation IF(Human renal epithelial cell line)
WB(Homo sapiens)
IHC(Mus musculus)
IF(Mus musculus)
WB(Mus musculus)
Western blot analysis of extracts of various cell lines, using DNA Polymerase beta antibody (A1681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and DNA Polymerase beta knockout (KO) HeLa cells, using DNA Polymerase beta antibody (A1681) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human stomach using DNA Polymerase beta antibody (A1681) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using DNA Polymerase beta antibody (A1681) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of U2OS cells using DNA Polymerase beta antibody (A1681). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human DNA Polymerase beta (NP_002681.1). KO Validated Validated Isotype IgG