![[KO Validated] Annexin A5/Annexin V Rabbit pAb (A1728)](/upload/resize_cache/iblock/f31/340_340_140cd750bba9870f18aada2478b24840a/2hvi31ffd6o7q1dh2shkfjaehifq0ak0.jpg "[KO Validated] Annexin A5/Annexin V Rabbit pAb (A1728)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Annexin A5/Annexin V Rabbit pAb (A1728)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Annexin A5/Annexin V Rabbit pAb Catalog No. A1728 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The Annexin 5 gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. Polymorphisms in this gene have been implicated in various obstetric complications.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human Annexin A5/Annexin V (NP_001145.1). Sequence MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD Gene ID Swiss Prot Synonyms PP4; ANX5; ENX2; CPB-I; RPRGL3; HEL-S-7; VAC-alph Calculated MW 36kDa Observed MW 35kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HepG2, HeLa, DU145, PC-3, Mouse lung Cellular location cytoplasm, cytosol, external side of plasma membrane, extracellular exosome, extracellular region, focal adhesion Customer validation IHC(Human prostate)
Western blot analysis of extracts of various cell lines, using Annexin A5/Annexin V antibody (A1728) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and Annexin A5/Annexin V knockout (KO) 293T cells, using Annexin A5/Annexin V antibody (A1728) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using [KO Validated] Annexin A5/Annexin V Rabbit pAb (A1728) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human Annexin A5/Annexin V (NP_001145.1). KO Validated Validated Isotype IgG