![[KO Validated] Lamin A/C Rabbit pAb (A17319)](/upload/resize_cache/iblock/4ac/340_340_140cd750bba9870f18aada2478b24840a/xumxl37bgu3q0fg4k30crnvd1xn8ccmz.jpg "[KO Validated] Lamin A/C Rabbit pAb (A17319)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Lamin A/C Rabbit pAb (A17319)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Lamin A/C Rabbit pAb Catalog No. A17319 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1). Sequence SSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSS Gene ID Swiss Prot Synonyms FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B Calculated MW 74kDa Observed MW 63kDa/74kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:200 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples HeLa, K-562, A-549 Cellular location Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm Customer validation WB(Mus musculus)
Western blot analysis of extracts of various cell lines, using Lamin A/C Rabbit pAb (A17319).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Western blot analysis of extracts from normal (control) and Lamin A/C Rabbit pAb knockout (KO) HeLa cells, using Lamin A/C Rabbit pAb antibody (A17319).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Immunohistochemistry analysis of paraffin-embedded mouse brain using [KO Validated] Lamin A/C Rabbit pAb (A17319) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using [KO Validated] Lamin A/C Rabbit pAb (A17319) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat liver using [KO Validated] Lamin A/C Rabbit pAb (A17319) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using Lamin A/C antibody (A17319) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Lamin A/C antibody (A17319) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Lamin A/C antibody (A17319) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of K-562 cells using 3 μg Lamin A/C antibody (A17319). Western blot was performed from the immunoprecipitate using Lamin A/C antibody (A17319) at a dilution of 1:1000.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1). KO Validated Validated Isotype IgG