![[KO Validated] EDN1 Rabbit pAb (A18004)](/upload/resize_cache/iblock/5f1/340_340_140cd750bba9870f18aada2478b24840a/xtk71fexvnn9hecw75zg8r2hlrxf9a2q.jpg "[KO Validated] EDN1 Rabbit pAb (A18004)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] EDN1 Rabbit pAb (A18004)
- Описание
- Характеристики
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Overview
Product name [KO Validated] EDN1 Rabbit pAb Catalog No. A18004 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a preproprotein that is proteolytically processed to generate a secreted peptide that belongs to the endothelin/sarafotoxin family. This peptide is a potent vasoconstrictor and its cognate receptors are therapeutic targets in the treatment of pulmonary arterial hypertension. Aberrant expression of this gene may promote tumorigenesis. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 18-212 of human EDN1 (NP_001946.3). Sequence APETAVLGAELSAVGENGGEKPTPSPPWRLRRSKRCSCSSLMDKECVYFCHLDIIWVNTPEHVVPYGLGSPRSKRALENLLPTKATDRENRCQCASQKDKKCWNFCQAGKELRAEDIMEKDWNNHKKGKDCSKLGKKCIYQQLVRGRKIRRSSEEHLRQTRSETMRNSVKSSFHDPKLKGKPSRERYVTHNRAHW Gene ID Swiss Prot Synonyms ET1; QME; PPET1; ARCND3; HDLCQ7 Calculated MW 24kDa Observed MW 24kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa Cellular location Secreted 
Western blot analysis of extracts from normal (control) and EDN1 knockout (KO) HeLa cells, using EDN1 antibody (A18004) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 18-212 of human EDN1 (NP_001946.3). KO Validated Validated Isotype IgG