![[KO Validated] AKR1A1 Rabbit pAb (A18014)](/upload/resize_cache/iblock/f41/340_340_140cd750bba9870f18aada2478b24840a/75wm1hod6jtnhatdbxee3i6ez2agtwmv.jpg "[KO Validated] AKR1A1 Rabbit pAb (A18014)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] AKR1A1 Rabbit pAb (A18014)
- Описание
- Характеристики
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Overview
Product name [KO Validated] AKR1A1 Rabbit pAb Catalog No. A18014 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human AKR1A1 (NP_006057.1). Sequence MAASCVLLHTGQKMPLIGLGTWKSEPGQVKAAVKYALSVGYRHIDCAAIYGNEPEIGEALKEDVGPGKAVPREELFVTSKLWNTKHHPEDVEPALRKTLADLQLEYLDLYLMHWPYAFERGDNPFPKNADGTICYDSTHYKETWKALEALVAKGLVQALGLSNFNSRQIDDILSVASVRPAVLQVECHPYLAQNELIAHCQARGLEVTAYSPLGSSDRAWRDPDEPVLLEEPVVLALAEKYGRSPAQILLRWQVQRKVICIPKSITPSRILQNIKVFDFTFSPEEMKQLNALNKNWRYIVPMLTVDGKRVPRDAGHPLYPFNDPY Gene ID Swiss Prot Synonyms ALR; ARM; DD3; ALDR1; HEL-S-6 Calculated MW 37kDa Observed MW 36kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location apical plasma membrane, cytosol, extracellular exosome, extracellular space 
Western blot analysis of extracts from normal (control) and AKR1A1 knockout (KO) 293T cells, using AKR1A1 antibody (A18014) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human AKR1A1 (NP_006057.1). KO Validated Validated Isotype IgG