![[KO Validated] Hsp90β Rabbit pAb (A18015)](/upload/resize_cache/iblock/7d9/340_340_140cd750bba9870f18aada2478b24840a/sefllptvqba0r4ud99vcr1rklb5g3hw2.jpg "[KO Validated] Hsp90β Rabbit pAb (A18015)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Hsp90β Rabbit pAb (A18015)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Hsp90β Rabbit pAb Catalog No. A18015 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90β (NP_031381.2). Sequence MPEEVHHGEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNASDALDKIRYESLTDPSKLDSGKELKIDIIPNPQERTLTLVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVVVITKHNDDEQYAWESSAGGSFTVRADHGEPIGRGTKVILHLKEDQTEYLEERRVKEVVKKHSQFIGYPITLYLEKEREKEISDDEAEEEKGEKEEEDKDDEEKPKIEDVGSDEEDDSGKDKKKKTKKIKEKYIDQEELNKTKPIWTRNPDDITQEE Gene ID Swiss Prot Synonyms HSP84; HSPC2; HSPCB; D6S182; HSP90B Calculated MW 83kDa Observed MW 80kDa Applications
Reactivity Human, Mouse, Rat, Monkey Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa Cellular location Cytoplasm, Melanosome 
Western blot analysis of extracts from normal (control) and HSP90AB1 knockout (KO) HeLa cells, using HSP90AB1 antibody (A18015) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Обезьяна Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Hsp90β (NP_031381.2). KO Validated Validated Isotype IgG