![[KO Validated] EIF3H Rabbit pAb (A18026)](/upload/resize_cache/iblock/a23/340_340_140cd750bba9870f18aada2478b24840a/a9akjuniib9p3xuztlv509y7woti9zgl.jpg "[KO Validated] EIF3H Rabbit pAb (A18026)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] EIF3H Rabbit pAb (A18026)
- Описание
- Характеристики
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Overview
Product name [KO Validated] EIF3H Rabbit pAb Catalog No. A18026 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables deubiquitinase activity. Contributes to translation initiation factor activity. Involved in negative regulation of proteasomal ubiquitin-dependent protein catabolic process and translational initiation. Located in extracellular exosome and membrane. Part of eukaryotic translation initiation factor 3 complex. Implicated in breast cancer; prostate cancer; and prostate carcinoma. Biomarker of prostate cancer.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1). Sequence NCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWYQSTYYGSFVTRALLDSQFSYQHAIEESVVLIYDPIKTAQGSLSLKAYRLTPKLMEVCKEKDFSPEALKKANITFEYMFEEVPIVIKNSHLINVLMWELEKKSAVADKHELLSLASSNHLGKNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEEDLSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGK Gene ID Swiss Prot Synonyms EIF3S3; eIF3-p40; eIF3-gamma Calculated MW 40kDa Observed MW 40kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HeLa Cellular location Cytoplasm 
Western blot analysis of extracts from normal (control) and EIF3H knockout (KO) HeLa cells, using EIF3H antibody (A18026) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human EIF3H (NP_003747.1). KO Validated Validated Isotype IgG