![[KO Validated] BUB1 Rabbit pAb (A18053)](/upload/resize_cache/iblock/864/340_340_140cd750bba9870f18aada2478b24840a/pssyo6qbj5sngnfc2gc77quf7bhff3ev.jpg "[KO Validated] BUB1 Rabbit pAb (A18053)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] BUB1 Rabbit pAb (A18053)
- Описание
- Характеристики
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Overview
Product name [KO Validated] BUB1 Rabbit pAb Catalog No. A18053 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BUB1 (NP_004327.1). Sequence MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVEENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFISYCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAWAGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQYRLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSKSNPGNNMACISKNQGSELSGVISSACDKESNMERRVITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRGESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKEANAFEEQLLKQKMDELHKKL Gene ID Swiss Prot Synonyms BUB1A; BUB1L; hBUB1; MCPH30 Calculated MW 122kDa Observed MW 133kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Chromosome, Nucleus, centromere, kinetochore 
Western blot analysis of extracts from normal (control) and BUB1 knockout (KO) HeLa cells, using BUB1 antibody (A18053) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1min. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BUB1 (NP_004327.1). KO Validated Validated Isotype IgG