![[KO Validated] GSK3α Rabbit pAb (A18655)](/upload/resize_cache/iblock/824/340_340_140cd750bba9870f18aada2478b24840a/56151s20iw1y3w2t5pv664a3ah8cdsvw.jpg "[KO Validated] GSK3α Rabbit pAb (A18655)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] GSK3α Rabbit pAb (A18655)
- Описание
- Характеристики
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Overview
Product name [KO Validated] GSK3α Rabbit pAb Catalog No. A18655 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 400-483 of human GSK3α (NP_063937.2). Sequence HSFFDELRCLGTQLPNNRPLPPLFNFSAGELSIQPSLNAILIPPHLRSPAGTTTLTPSSQALTETPTSSDWQSTDATPTLTNSS Gene ID Swiss Prot Synonyms GSK3A; glycogen synthase kinase-3 alpha Calculated MW 51kDa Observed MW 51kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, U-251MG, Mouse brain, Rat brain Cellular location apical dendrite, axon, beta-catenin destruction complex, cytoplasm, cytosol, mitochondrion, nucleus, postsynapse, proximal dendrite 
Western blot analysis of extracts from normal (control) and GSK3α knockout (KO) HeLa cells, using GSK3α antibody (18655) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot analysis of extracts of various cell lines, using GSK3α antibody (A18655) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 400-483 of human GSK3α (NP_063937.2). KO Validated Validated Isotype IgG