![[KO Validated] CDKN1A/p21 Rabbit mAb (A19094)](/upload/resize_cache/iblock/89c/340_340_140cd750bba9870f18aada2478b24840a/x3t0yqgvzd3eecfp43h57oir5hp11qbk.jpg "[KO Validated] CDKN1A/p21 Rabbit mAb (A19094)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] CDKN1A/p21 Rabbit mAb (A19094)
- Описание
- Характеристики
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Overview
Product name [KO Validated] CDKN1A/p21 Rabbit mAb Catalog No. A19094 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC51034 Background
This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-cyclin-dependent kinase2 or -cyclin-dependent kinase4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen, a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of cyclin-dependent kinase2, and may be instrumental in the execution of apoptosis following caspase activation. Mice that lack this gene have the ability to regenerate damaged or missing tissue. Multiple alternatively spliced variants have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1). Sequence MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEARERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQGTAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRKP Gene ID Swiss Prot Synonyms P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1 Calculated MW 18kDa Observed MW 21kDa/18kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples MCF7 cells, HeLa cells, 293T cells, Mouse large intestine, Mouse testis, Rat lung Cellular location Cytoplasm, Nucleus Customer validation WB(Homo sapiens, Rattus norvegicus, Mus musculus , Mus musculus)
WB，IHC(Homo sapiens)
IHC(Homo sapiens)
IF(Homo sapiens, Rattus norvegicus)
IHC(Homo sapiens)
IF(Homo sapiens, Mus musculus )
RT-qPCR(Homo sapiens)
IF(Homo sapiens, Mus musculus)
Western blot analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody (A19094) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 3min.
Western blot analysis of extracts of various cell lines, using CDKN1A/p21CIP1 antibody (A19094) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Western blot analysis of extracts from wild type(WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb (A19094) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver cancer using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat liver using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat ovary using [KO Validated] CDKN1A/p21CIP1 Rabbit mAb (A19094) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human CDKN1A/p21CIP1 (NP_000380.1). KO Validated Validated Isotype IgG