![[KO Validated] Lamin A/C Rabbit mAb (A19524)](/upload/resize_cache/iblock/331/340_340_140cd750bba9870f18aada2478b24840a/ltitrh9j9mhaer2c1z2rmza5nftk6gz1.jpg "[KO Validated] Lamin A/C Rabbit mAb (A19524)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] Lamin A/C Rabbit mAb (A19524)
- Описание
- Характеристики
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Overview
Product name [KO Validated] Lamin A/C Rabbit mAb Catalog No. A19524 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC5001-08 Background
The protein encoded by this gene is part of the nuclear lamina, a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1). Sequence SSHSSQTQGGGSVTKKRKLESTESRSSFSQHARTSGRVAVEEVDEEGKFVRLRNKSNEDQSMGNWQIKRQNGDDPLLTYRFPPKFTLKAGQVVTIWAAGAGATHSPPTDLVWKAQNTWGCGNSLRTALINSTGEEVAMRKLVRSVTVVEDDEDEDGDDLLHHHHGSHCSS Gene ID Swiss Prot Synonyms FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B Calculated MW 74kDa Observed MW 68kDa/72kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:10000 - 1:600000
- IHC-P 1:50 - 1:400
- IF/ICC 1:50 - 1:200
- IP 1:10000 - 1:120000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples HeLa, A-549, NIH/3T3, PC-12 Cellular location Nucleus, Nucleus envelope, Nucleus lamina, Nucleus speckle, nucleoplasm Customer validation (Caenorhabditis Elegans)
WB(Mus musculus, C. elegans)
IF(Homo sapiens)
WB(Homo sapiens)
Western Blot(Mus musculus)
Western blot analysis of extracts from wild type (WT) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody (A19524) at 1:50000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using [KO Validated] Lamin A/C Rabbit mAb antibody (A19524) at 1:500000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Western blot analysis of various lysates, using [KO Validated] Lamin A/C Rabbit mAb (A19524) at 1:300000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Lamin A/C antibody (A19524) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Lamin A/C antibody (A19524) at dilution of 1:400 - 1:3200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of H9C2 cells using Lamin A/C antibody (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of L929 cells using Lamin A/C antibody (A19524) at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of HeLa cells using Lamin A/C antibody (A19524) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using Lamin A/C antibody (A19524) at dilution of 1:100 - 1:1600. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts from PC-12 cells using 3 μg [KO Validated] Lamin A/C Rabbit mAb (A19524). Western blot was performed from the immunoprecipitate using [KO Validated] Lamin A/C Rabbit mAb (A19524) at a dilution of 1:100000.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin A/C (NP_733821.1). KO Validated Validated Isotype IgG