![[KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)](/upload/resize_cache/iblock/aa0/340_340_140cd750bba9870f18aada2478b24840a/amvku7cdb58ibs6721awoxtvcx4rdy9r.jpg "[KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] HuR/ELAVL1 Rabbit mAb (A19622)
- Описание
- Характеристики
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Overview
Product name [KO Validated] HuR/ELAVL1 Rabbit mAb Catalog No. A19622 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC0101 Background
The protein encoded by this gene is a member of the ELAVL family of RNA-binding proteins that contain several RNA recognition motifs, and selectively bind AU-rich elements (AREs) found in the 3' untranslated regions of mRNAs. AREs signal degradation of mRNAs as a means to regulate gene expression, thus by binding AREs, the ELAVL family of proteins play a role in stabilizing ARE-containing mRNAs. This gene has been implicated in a variety of biological processes and has been linked to a number of diseases, including cancer. It is highly expressed in many cancers, and could be potentially useful in cancer diagnosis, prognosis, and therapy.Immunogen information
Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717). Sequence MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSS Gene ID Swiss Prot Synonyms HUR; Hua; MelG; ELAV1 Calculated MW 36kDa Observed MW 36kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples 293T, HeLa, Jurkat, Mouse brain, Mouse spleen, Mouse thymus, Rat brain Cellular location Cytoplasm, Nucleus Customer validation WB(Homo sapiens, Mus musculus)
IP(Homo sapiens)
Western blot analysis of extracts from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using HuR/ELAVL1 antibody (A19622) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using HuR/ELAVL1 antibody (A19622) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human tonsil using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (A19622, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg HuR/ELAVL1 antibody (A19622). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody (A19622) at a dilution of 1:1000.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HuR/ELAVL1 (Q15717). KO Validated Validated Isotype IgG