![[KO Validated] DNAJC9 Rabbit pAb (A19850)](/upload/resize_cache/iblock/1f2/340_340_140cd750bba9870f18aada2478b24840a/295muwdull5hy7dryjemcl17pzaqrj7z.jpg "[KO Validated] DNAJC9 Rabbit pAb (A19850)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] DNAJC9 Rabbit pAb (A19850)
- Описание
- Характеристики
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Overview
Product name [KO Validated] DNAJC9 Rabbit pAb Catalog No. A19850 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables chaperone binding activity; heat shock protein binding activity; and histone binding activity. Involved in nucleosome assembly and positive regulation of ATPase activity. Located in several cellular components, including cytosol; extracellular space; and nucleoplasm. Part of chaperone complex.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DNAJC9 (NP_056005.1). Sequence MGLLDLCEEVFGTADLYRVLGVRREASDGEVRRGYHKVSLQVHPDRVGEGDKEDATRRFQILGKVYSVLSDREQRAVYDEQGTVDEDSPVLTQDRDWEAYWRLLFKKISLEDIQAFEKTYKGSEEELADIKQAYLDFKGDMDQIMESVLCVQYTEEPRIRNIIQQAIDAGEVPSYNAFVKESKQKMNARKRRAQEEAKEAEMSRKELGLDEGVDSLKAAIQSRQKDRQKEMDNFLAQMEAKYCKSSKGGGKKSALKKEKK Gene ID Swiss Prot Synonyms JDD1; SB73; HDJC9 Calculated MW 30kDa Observed MW 33kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Cell membrane, Cytoplasm, Nucleus 
Western blot analysis of extracts from normal (control) and DNAJC9 knockout (KO) 293T cells, using DNAJC9 antibody (A19850) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human DNAJC9 (NP_056005.1). KO Validated Validated Isotype IgG