![[KO Validated] NRBF2 Rabbit pAb (A19856)](/upload/resize_cache/iblock/52e/340_340_140cd750bba9870f18aada2478b24840a/6qzi9agi29o6a30m2fng0klofb4cl9o5.jpg "[KO Validated] NRBF2 Rabbit pAb (A19856)")
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Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] NRBF2 Rabbit pAb (A19856)
- Описание
- Характеристики
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Overview
Product name [KO Validated] NRBF2 Rabbit pAb Catalog No. A19856 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Involved in autophagy. Located in cytoplasm. Colocalizes with phosphatidylinositol 3-kinase complex, class III.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 10-220 of human NRBF2 (NP_110386.2). Sequence LAHQQSRRADRLLAAGKYEEAISCHKKAAAYLSEAMKLTQSEQAHLSLELQRDSHMKQLLLIQERWKRAQREERLKAQQNTDKDAAAHLQTSHKPSAEDAEGQSPLSQKYSPSTEKCLPEIQGIFDRDPDTLLYLLQQKSEPAEPCIGSKAPKDDKTIIEEQATKIADLKRHVEFLVAENERLRKENKQLKAEKARLLKGPIEKELDVDAD Gene ID Swiss Prot Synonyms COPR; COPR1; COPR2; NRBF-2 Calculated MW 32kDa Observed MW 40kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Cytoplasm, Cytoplasmic vesicle, Nucleus, autophagosome 
Western blot analysis of extracts from normal (control) and NRBF2 knockout (KO) HeLa cells, using NRBF2 antibody (A19856) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 10-220 of human NRBF2 (NP_110386.2). KO Validated Validated Isotype IgG