![[KO Validated] KIF22 Rabbit pAb (A19881)](/upload/resize_cache/iblock/547/340_340_140cd750bba9870f18aada2478b24840a/56l3ze2hvu4byok85hren4ecqs5b3h42.jpg "[KO Validated] KIF22 Rabbit pAb (A19881)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] KIF22 Rabbit pAb (A19881)
- Описание
- Характеристики
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Overview
Product name [KO Validated] KIF22 Rabbit pAb Catalog No. A19881 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a member of the kinesin-like protein family. The family members are microtubule-dependent molecular motors that transport organelles within cells and move chromosomes during cell division. The C-terminal half of this protein has been shown to bind DNA. Studies with the Xenopus homolog suggests its essential role in metaphase chromosome alignment and maintenance. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 456-665 of human KIF22 (NP_015556.1). Sequence QGAPLLSTPKRERMVLMKTVEEKDLEIERLKTKQKELEAKMLAQKAEEKENHCPTMLRPLSHRTVTGAKPLKKAVVMPLQLIQEQAASPNAEIHILKNKGRKRKLESLDALEPEEKAEDCWELQISPELLAHGRQKILDLLNEGSARDLRSLQRIGPKKAQLIVGWRELHGPFSQVEDLERVEGITGKQMESFLKANILGLAAGQRCGAS Gene ID Swiss Prot Synonyms KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2 Calculated MW 73kDa Observed MW 73kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:200 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Cytoplasm, Nucleus, cytoskeleton Customer validation IF(Homo sapiens)
Western blot analysis of extracts from normal (control) and KIF22 knockout (KO) 293T cells, using KIF22 antibody (A19881) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 456-665 of human KIF22 (NP_015556.1). KO Validated Validated Isotype IgG