![[KO Validated] GSTO1 Rabbit pAb (A19892)](/upload/resize_cache/iblock/138/340_340_140cd750bba9870f18aada2478b24840a/zd55512yex4vnzb30t9g88c6y4lrv0br.jpg "[KO Validated] GSTO1 Rabbit pAb (A19892)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] GSTO1 Rabbit pAb (A19892)
- Описание
- Характеристики
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Overview
Product name [KO Validated] GSTO1 Rabbit pAb Catalog No. A19892 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human GSTO1 (NP_004823.1). Sequence MSGESARSLGKGSAPPGPVPEGSIRIYSMRFCPFAERTRLVLKAKGIRHEVININLKNKPEWFFKKNPFGLVPVLENSQGQLIYESAITCEYLDEAYPGKKLLPDDPYEKACQKMILELFSKVPSLVGSFIRSQNKEDYAGLKEEFRKEFTKLEEVLTNKKTTFFGGNSISMIDYLIWPWFERLEAMKLNECVDHTPKLKLWMAAMKEDPTVSALLTSEKDWQGFLELYLQNSPEACDYGL Gene ID Swiss Prot Synonyms P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21 Calculated MW 28kDa Observed MW 27kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:200 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Cytoplasm, cytosol 
Western blot analysis of extracts from normal (control) and GSTO1 knockout (KO) 293T cells, using GSTO1 antibody (A19892) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-241 of human GSTO1 (NP_004823.1). KO Validated Validated Isotype IgG