![[KO Validated] M6PR Rabbit pAb (A19907)](/upload/resize_cache/iblock/129/340_340_140cd750bba9870f18aada2478b24840a/tl29b6lbljddwtdwgfvggl1qx428sm35.jpg "[KO Validated] M6PR Rabbit pAb (A19907)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] M6PR Rabbit pAb (A19907)
- Описание
- Характеристики
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Overview
Product name [KO Validated] M6PR Rabbit pAb Catalog No. A19907 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the P-type lectin family. P-type lectins play a critical role in lysosome function through the specific transport of mannose-6-phosphate-containing acid hydrolases from the Golgi complex to lysosomes. The encoded protein functions as a homodimer and requires divalent cations for ligand binding. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. A pseudogene of this gene is located on the long arm of chromosome X.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 27-185 of human M6PR (NP_002346.1). Sequence TEEKTCDLVGEKGKESEKELALVKRLKPLFNKSFESTVGQGSDTYIYIFRVCREAGNHTSGAGLVQINKSNGKETVVGRLNETHIFNGSNWIMLIYKGGDEYDNHCGKEQRRAVVMISCNRHTLADNFNPVSEERGKVQDCFYLFEMDSSLACSPEISH Gene ID Swiss Prot Synonyms SMPR; MPR46; CD-MPR; MPR 46; MPR-46; CD-M6PR Calculated MW 31kDa Observed MW 31kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Lysosome membrane, Single-pass type I membrane protein Customer validation WB(Homo sapiens)
IHC(Homo sapiens)
Western blot analysis of extracts from normal (control) and M6PR knockout (KO) 293T cells, using M6PR antibody (A19907) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 27-185 of human M6PR (NP_002346.1). KO Validated Validated Isotype IgG