![[KO Validated] HSP105/HSPH1 Rabbit pAb (A19914)](/upload/resize_cache/iblock/0e2/340_340_140cd750bba9870f18aada2478b24840a/0r5b37ops0v0rs309pojhz14jci781y9.jpg "[KO Validated] HSP105/HSPH1 Rabbit pAb (A19914)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] HSP105/HSPH1 Rabbit pAb (A19914)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] HSP105/HSPH1 Rabbit pAb Catalog No. A19914 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 659-858 of human HSP105/HSP105/HSPH1 (NP_006635.2). Sequence EQDHQNFLRLLTETEDWLYEEGEDQAKQAYVDKLEELMKIGTPVKVRFQEAEERPKMFEELGQRLQHYAKIAADFRNKDEKYNHIDESEMKKVEKSVNEVMEWMNNVMNAQAKKSLDQDPVVRAQEIKTKIKELNNTCEPVVTQPKPKIESPKLERTPNGPNIDKKEEDLEDKNNFGAEPPHQNGECYPNEKNSVNMDLD Gene ID Swiss Prot Synonyms HSP105; HSP105A; HSP105B; NY-CO-25 Calculated MW 97kDa Observed MW 97kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Cytoplasm 
Western blot analysis of extracts from normal (control) and HSP105/HSP105/HSPH1 knockout (KO) 293T cells, using HSP105/HSP105/HSPH1 antibody (A19914) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 659-858 of human HSP105/HSP105/HSPH1 (NP_006635.2). KO Validated Validated Isotype IgG