![[KO Validated] AES Rabbit pAb (A19944)](/upload/resize_cache/iblock/15c/340_340_140cd750bba9870f18aada2478b24840a/bqnt71jl6eeco5ezc846f88xuvy4f04a.jpg "[KO Validated] AES Rabbit pAb (A19944)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] AES Rabbit pAb (A19944)
- Описание
- Характеристики
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Overview
Product name [KO Validated] AES Rabbit pAb Catalog No. A19944 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is similar in sequence to the amino terminus of Drosophila enhancer of split groucho, a protein involved in neurogenesis during embryonic development. The encoded protein, which belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. Three transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human AES (NP_945320.1). Sequence MCHKNGFPQEGGITAAFLQKRKLRLSKNHRPARAKVTEHVRGTRPGRATAGPAASTRAAGSLFFDRWGNRGPAGCRGSSHLPQQLKFTTSDSCDRIKDEFQLLQAQYHSLKLECDKLASEKSEMQRHYVMYYEMSYGLNIEMHKQAEIVKRLNGICAQVLPYLSQEHQQQVLGAIERAKQVTAPELNSIIRQQLQAHQLSQLQALALPLTPLPVGLQPPSLPAVSAGTGLLSLSALGSQAHLSKEDKNGHDGDTHQEDDGEKSD Gene ID Swiss Prot Synonyms AES; GRG; ESP1; GRG5; AES-1; AES-2; Grg-5 Calculated MW 22kDa Observed MW 22kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Nucleus 
Western blot analysis of extracts from normal (control) and AES knockout (KO) HeLa cells, using AES antibody (A19944) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human AES (NP_945320.1). KO Validated Validated Isotype IgG