![[KO Validated] HMG20B Rabbit pAb (A19951)](/upload/resize_cache/iblock/120/340_340_140cd750bba9870f18aada2478b24840a/43cui9sz3hcaq1qyy9d8hv5rxjfg7q8f.jpg "[KO Validated] HMG20B Rabbit pAb (A19951)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] HMG20B Rabbit pAb (A19951)
- Описание
- Характеристики
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Overview
Product name [KO Validated] HMG20B Rabbit pAb Catalog No. A19951 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Predicted to enable DNA binding activity. Predicted to be involved in regulation of gene expression. Predicted to act upstream of or within negative regulation of protein sumoylation; positive regulation of neuron differentiation; and skeletal muscle cell differentiation. Located in nuclear body.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human HMG20B (NP_006330.2). Sequence MSHGPKQPGAAAAPAGGKAPGQHGGFVVTVKQERGEGPRAGEKGSHEEEPVKKRGWPKGKKRKKILPNGPKAPVTGYVRFLNERREQIRTRHPDLPFPEITKMLGAEWSKLQPTEKQRYLDEAEREKQQYMKELRAYQQSEAYKMCTEKIQEKKIKKEDSSSGLMNTLLNGHKGGDCDGFSTFDVPIFTEEFLDQNKAREAELRRLRKMNVAFEEQNAVLQRHTQSMSSARERLEQELALEERRTLALQQQLQAVRQALTASFASLPVPGTGETPTLGTLDFYMARLHGAIERDPAQHEKLIVRIKEILAQVASEHL Gene ID Swiss Prot Synonyms SOXL; HMGX2; BRAF25; BRAF35; HMGXB2; PP7706; pp8857; SMARCE1r Calculated MW 36kDa Observed MW 36kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:200 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Chromosome, Nucleus 
Western blot analysis of extracts from normal (control) and HMG20B knockout (KO) 293T cells, using HMG20B antibody (A19951) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-317 of human HMG20B (NP_006330.2). KO Validated Validated Isotype IgG