![[KO Validated] NHEJ1 Rabbit pAb (A19957)](/upload/resize_cache/iblock/067/340_340_140cd750bba9870f18aada2478b24840a/n2270tfbfmme6yetgawgdaso5x858e1w.jpg "[KO Validated] NHEJ1 Rabbit pAb (A19957)")
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Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] NHEJ1 Rabbit pAb (A19957)
- Описание
- Характеристики
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Overview
Product name [KO Validated] NHEJ1 Rabbit pAb Catalog No. A19957 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1). Sequence MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQVWHEQVDTSVVSQRAKELNKRLTAPPAAFLCHLDNLLRPLLKDAAHPSEATFSCDCVADALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLMGMSLALQCQVRELATLLHMKDLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPFVMNLQDLYMAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTGTSGPLQRPQLSKVKRKKPRGLFS Gene ID Swiss Prot Synonyms XLF Calculated MW 33kDa Observed MW 39kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:200 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Nucleus Customer validation WB(Homo sapiens)
Western blot analysis of extracts from normal (control) and NHEJ1 knockout (KO) HeLa cells, using NHEJ1 antibody (A19957) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1). KO Validated Validated Isotype IgG