![[KO Validated] GSTK1 Rabbit pAb (A19963)](/upload/resize_cache/iblock/e13/340_340_140cd750bba9870f18aada2478b24840a/rl99rm76f03on54vebybmeef1en2e4g6.jpg "[KO Validated] GSTK1 Rabbit pAb (A19963)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] GSTK1 Rabbit pAb (A19963)
- Описание
- Характеристики
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Overview
Product name [KO Validated] GSTK1 Rabbit pAb Catalog No. A19963 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a member of the kappa class of the glutathione transferase superfamily of enzymes that function in cellular detoxification. The encoded protein is localized to the peroxisome and catalyzes the conjugation of glutathione to a wide range of hydrophobic substates facilitating the removal of these compounds from cells. Alternative splicing results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1). Sequence MGPLPRTVELFYDVLSPYSWLGFEILCRYQNIWNINLQLRPSLITGIMKDSGNKPPGLLPRKGLYMANDLKLLRHHLQIPIHFPKDFLSVMLEKGSLSAMRFLTAVNLEHPEMLEKASRELWMRVWSRNEDITEPQSILAAAEKAGMSAEQAQGLLEKIATPKVKNQLKETTEAACRYGAFGLPITVAHVDGQTHMLFGSDRMELLAHLLGEKWMGPIPPAVNARL Gene ID Swiss Prot Synonyms GST; GST13; hGSTK1; GSTK1-1; GST13-13; GST 13-13 Calculated MW 25kDa Observed MW 23kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Peroxisome Customer validation WB(Mus musculus)
Western blot analysis of extracts from normal (control) and GSTK1 knockout (KO) 293T cells, using GSTK1 antibody (A19963) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-226 of human GSTK1 (NP_057001.1). KO Validated Validated Isotype IgG