![[KO Validated] HPCAL1 Rabbit pAb (A19964)](/upload/resize_cache/iblock/d11/340_340_140cd750bba9870f18aada2478b24840a/aoj3dj80myy7jgne6zp12zlct21brlj0.jpg "[KO Validated] HPCAL1 Rabbit pAb (A19964)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] HPCAL1 Rabbit pAb (A19964)
- Описание
- Характеристики
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Overview
Product name [KO Validated] HPCAL1 Rabbit pAb Catalog No. A19964 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Several alternatively spliced transcript variants encoding the same protein have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human HPCAL1 (NP_002140.2). Sequence MGKQNSKLRPEVLQDLRENTEFTDHELQEWYKGFLKDCPTGHLTVDEFKKIYANFFPYGDASKFAEHVFRTFDTNGDGTIDFREFIIALSVTSRGKLEQKLKWAFSMYDLDGNGYISRSEMLEIVQAIYKMVSSVMKMPEDESTPEKRTDKIFRQMDTNNDGKLSLEEFIRGAKSDPSIVRLLQCDPSSASQF Gene ID Swiss Prot Synonyms BDR1; HLP2; VILIP-3 Calculated MW 22kDa Observed MW 18kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IP Recommended dilution - WB 1:500 - 1:2000
- IP 1:20 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples HeLa Cellular location Lipid-anchor, Membrane 
Western blot analysis of extracts from normal (control) and HPCAL1 knockout (KO) HeLa cells, using HPCAL1 antibody (A19964) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human HPCAL1 (NP_002140.2). KO Validated Validated Isotype IgG