![[KO Validated] LAMTOR1 Rabbit pAb (A19983)](/upload/resize_cache/iblock/860/340_340_140cd750bba9870f18aada2478b24840a/acww3xvfjw98vgf1kxddfrw3sek2kqct.jpg "[KO Validated] LAMTOR1 Rabbit pAb (A19983)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] LAMTOR1 Rabbit pAb (A19983)
- Описание
- Характеристики
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Overview
Product name [KO Validated] LAMTOR1 Rabbit pAb Catalog No. A19983 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables GTPase binding activity. Contributes to guanyl-nucleotide exchange factor activity and molecular adaptor activity. Involved in several processes, including cholesterol homeostasis; positive regulation of TOR signaling; and regulation of cholesterol transport. Located in lysosome. Part of Ragulator complex.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LAMTOR1 (NP_060377.1). Sequence MGCCYSSENEDSDQDREERKLLLDPSSPPTKALNGAEPNYHSLPSARTDEQALLSSILAKTASNIIDVSAADSQGMEQHEYMDRARQYSTRLAVLSSSLTHWKKLPPLPSLTSQPHQVLASEPIPFSDLQQVSRIAAYAYSALSQIRVDAKEELVVQFGIP Gene ID Swiss Prot Synonyms p18; PDRO; C11orf59; p27RF-Rho; Ragulator1 Calculated MW 18kDa Observed MW 18kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa Cellular location Cell membrane, Cytoplasmic side, Late endosome membrane, Lipid-anchor, Lysosome membrane 
Western blot analysis of extracts from normal (control) and LAMTOR1 knockout (KO) HeLa cells, using LAMTOR1 antibody (A19983) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human LAMTOR1 (NP_060377.1). KO Validated Validated Isotype IgG