![[KO Validated] LAMP1 Rabbit mAb (A21194)](/upload/resize_cache/iblock/9ff/340_340_140cd750bba9870f18aada2478b24840a/c7iyprh22fpupi15rcltl0sb4pbwqs69.jpg "[KO Validated] LAMP1 Rabbit mAb (A21194)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] LAMP1 Rabbit mAb (A21194)
- Описание
- Характеристики
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Overview
Product name [KO Validated] LAMP1 Rabbit mAb Catalog No. A21194 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC52154 Background
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-382 of human LAMP1 (NP_005552.3). Sequence AMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSM Gene ID Swiss Prot Synonyms LAMPA; CD107a; LGP120 Calculated MW 45kDa Observed MW 42kDa, 90-120kDa Applications
Reactivity Human Tested applications WB IHC-P IF/ICC FC Recommended dilution - WB 1:2000 - 1:10000
- IHC-P 1:500 - 1:1000
- IF/ICC 1:500 - 1:1000
- FC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Flow Cytometry Positive samples U-251 MG, HeLa Cellular location cytoplasm, cytosol, external side of plasma membrane, extracellular exosome, late endosome, lysosome, melanosome, multivesicular body, perinuclear region of cytoplasm, plasma membrane 
Western blot analysis of extracts of U-251MG cells, using LAMP1 antibody (A21194) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Western blot analysis of extracts from wild type (WT) and LAMP1 knockout (KO) HeLa, using LAMP1 antibody (A21194) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
Immunohistochemistry analysis of paraffin-embedded human kidney using LAMP1 Rabbit mAb (A21194) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of wild type (WT) and LAMP1 knockout (KO) HeLa cells using [KO Validated] LAMP1 Rabbit mAb (A21194) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Flow Cytometry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 29-382 of human LAMP1 (NP_005552.3). KO Validated Validated Isotype IgG