![[KO Validated] 14-3-3 sigma Rabbit pAb (A21438)](/upload/resize_cache/iblock/2ff/340_340_140cd750bba9870f18aada2478b24840a/4kym2qiz1f0g262jyjj9m1nftw8g9cst.jpg "[KO Validated] 14-3-3 sigma Rabbit pAb (A21438)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] 14-3-3 sigma Rabbit pAb (A21438)
- Описание
- Характеристики
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Overview
Product name [KO Validated] 14-3-3 sigma Rabbit pAb Catalog No. A21438 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human 14-3-3 sigma (NP_006133.1). Sequence MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS Gene ID Swiss Prot Synonyms YWHAS Calculated MW 28kDa Observed MW 27kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa Cellular location Cytoplasm, Nucleus, Secreted 
Western blot analysis of extracts from wild type(WT) and 14-3-3 sigma knockout (KO) HeLa(KO) cells, using 14-3-3 sigma antibody (A21438) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-248 of human 14-3-3 sigma (NP_006133.1). KO Validated Validated Isotype IgG