![[KO Validated] MSH2 Rabbit pAb (A21494)](/upload/resize_cache/iblock/1a2/340_340_140cd750bba9870f18aada2478b24840a/zd9s29x6qve1d0epydmykhka5wgmdndj.jpg "[KO Validated] MSH2 Rabbit pAb (A21494)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] MSH2 Rabbit pAb (A21494)
- Описание
- Характеристики
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Overview
Product name [KO Validated] MSH2 Rabbit pAb Catalog No. A21494 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1). Sequence MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEVYKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQVGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQRGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNSAVLPEMENQVAVSSLSAVIKFLELLSDDSNFGQFELTTFDFSQYM Gene ID Swiss Prot Synonyms FCC1; COCA1; HNPCC; LCFS2; MSH-2; hMSH2; HNPCC1; LYNCH1; MMRCS2 Calculated MW 105kDa Observed MW 105kDa Applications
Reactivity Human, Rat Tested applications WB IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
- IP 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Immunoprecipitation Positive samples HeLa, A-549 Cellular location Nucleus 
Western blot analysis of A-549, using MSH2 Rabbit pAb antibody (A21494) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts from wild type(WT) and MSH2 Rabbit pAb knockout (KO) HeLa cells, using MSH2 Rabbit pAb antibody (A21494) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg MSH2 antibody (A21494). Western blot was performed from the immunoprecipitate using MSH2 antibody (A21494) at a dilution of 1:3000.
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Key applications Western blotting, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1). KO Validated Validated Isotype IgG