![[KO Validated] p53 Rabbit pAb (A21629)](/upload/resize_cache/iblock/9a3/340_340_140cd750bba9870f18aada2478b24840a/f3km89xkbzdtraw03jyqiizlffcedtwq.jpg "[KO Validated] p53 Rabbit pAb (A21629)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] p53 Rabbit pAb (A21629)
- Описание
- Характеристики
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Overview
Product name [KO Validated] p53 Rabbit pAb Catalog No. A21629 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).Immunogen information
Immunogen Recombinant protein of human p53. Sequence Email for sequence Gene ID Swiss Prot Synonyms P53; BCC7; LFS1; BMFS5; TRP53 Calculated MW 44kDa Observed MW 53kDa Applications
Reactivity Human Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:100 - 1:500
- IF/ICC 1:50 - 1:200
- IP 1:1000 - 1:5000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples 293T Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body 
Western blot analysis of extracts of 293T cells, using p53 Rabbit pAb (A21629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type (WT) and p53 knockout (KO) 293T cells, using p53 antibody (A21629) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using [KO Validated] p53 Rabbit pAb (A21629) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal immunofluorescence analysis of Hela cells using p53 Polyclonal Antibody (A21629) at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2 OS cells using p53 Polyclonal Antibody (A21629) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg p53 antibody (A21629). Western blot was performed from the immunoprecipitate using p53 antibody (A21629) at a dilution of 1:3000.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human p53. KO Validated Validated Isotype IgG