![[KO Validated] IGFBP5 Rabbit pAb (A21745)](/upload/resize_cache/iblock/7e8/340_340_140cd750bba9870f18aada2478b24840a/tr2sezbapwis4byycuhcrsqi735holau.jpg "[KO Validated] IGFBP5 Rabbit pAb (A21745)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] IGFBP5 Rabbit pAb (A21745)
- Описание
- Характеристики
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Overview
Product name [KO Validated] IGFBP5 Rabbit pAb Catalog No. A21745 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables insulin-like growth factor I binding activity. Involved in several processes, including cellular response to cAMP; regulation of smooth muscle cell migration; and regulation of smooth muscle cell proliferation. Part of insulin-like growth factor ternary complex. Biomarker of pulmonary fibrosis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-272 of human IGFBP5 (NP_000590.1). Sequence LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE Gene ID Swiss Prot Synonyms IBP5 Calculated MW 31kDa Observed MW 31kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, U2OS, BT-474 Cellular location Secreted 
Western blot analysis of extracts of various cell lines, using IGFBP5 antibody (A21745) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of extracts from wild type (WT) and IGFBP5 knockout (KO) 293T cells, using IGFBP5 antibody (A21745) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 21-272 of human IGFBP5 (NP_000590.1). KO Validated Validated Isotype IgG