![[KO Validated] MLH1 Mouse mAb (A21882)](/upload/resize_cache/iblock/6ab/340_340_140cd750bba9870f18aada2478b24840a/hx9gbxdzw3sne6yid220nm6sxaynmsyj.jpg "[KO Validated] MLH1 Mouse mAb (A21882)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] MLH1 Mouse mAb (A21882)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] MLH1 Mouse mAb Catalog No. A21882 Host species Mouse Purification method Affinity purification Isotype IgG1, Kappa CloneNo. AMC0417 Background
The protein encoded by this gene can heterodimerize with mismatch repair endonuclease PMS2 to form MutL alpha, part of the DNA mismatch repair system. When MutL alpha is bound by MutS beta and some accessory proteins, the PMS2 subunit of MutL alpha introduces a single-strand break near DNA mismatches, providing an entry point for exonuclease degradation. The encoded protein is also involved in DNA damage signaling and can heterodimerize with DNA mismatch repair protein MLH3 to form MutL gamma, which is involved in meiosis. This gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).Immunogen information
Immunogen Recombinant protein of human MLH1. Sequence Email for sequence Gene ID Swiss Prot Synonyms FCC2; COCA2; HNPCC; MLH-1; hMLH1; HNPCC2; LYNCH2; MMRCS1 Calculated MW 85kDa Observed MW Refer to figures Applications
Reactivity Human Tested applications WB IHC-P Recommended dilution - WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples Cellular location Nucleus 
Western blot analysis of extracts of Jurkat cells, using MLH1 Mouse mAb antibody (A21882) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of extracts from normal (control) and MLH1 Mouse mAb knockout (KO) HeLa cells, using MLH1 Mouse mAb antibody (A21882) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting, Immunohistochemistry Host species Мышь Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human MLH1. KO Validated Validated Isotype IgG1, Kappa