![[KO Validated] NG2/CSPG4 Rabbit pAb (A22012)](/upload/resize_cache/iblock/2c5/340_340_140cd750bba9870f18aada2478b24840a/t68by8ti0bkyuxue5rfcv33200uqgmuy.jpg "[KO Validated] NG2/CSPG4 Rabbit pAb (A22012)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] NG2/CSPG4 Rabbit pAb (A22012)
- Описание
- Характеристики
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Overview
Product name [KO Validated] NG2/CSPG4 Rabbit pAb Catalog No. A22012 Host species Rabbit Purification method Affinity purification Isotype IgG Background
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2). Sequence LGAPLEGVLVELEVLPAAIPLEAQNFSVPEGGSLTLAPPLLRVSGPYFPTLLGLSLQVLEPPQHGALQKEDGPQARTLSAFSWRMVEEQLIRYVHDGSETLTDSFVLMANASEMDRQSHPVAFTVTVLPVNDQPPILTTNTGLQMWEGATAPIPAEALRSTDGDSGSEDLVYTIEQPSNGRVVLRGAPGTEVRSFTQAQLDGGLVLFSHRGTLDGGFRFRLSDG Gene ID Swiss Prot Synonyms NG2; MCSP; MCSPG; MSK16; CSPG4A; HMW-MAA; MEL-CSPG Calculated MW 251kDa Observed MW 251kDa Applications
Reactivity Human, Mouse Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, MCF7, Mouse heart Cellular location apical plasma membrane, cell surface, extracellular exosome, extracellular region, focal adhesion, Golgi lumen, lamellipodium membrane, lysosomal lumen, nucleoplasm, plasma membrane 
Western blot analysis of various lysates, using NG2/CSPG4 Rabbit pAb antibody (A22012) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from wild type(WT) and NG2/CSPG4 Rabbit pAb knockout (KO) HeLa cells, using NG2/CSPG4 Rabbit pAb antibody (A22012) at 1:600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1340-1563 of human NG2/CSPG4 (NP_001888.2). KO Validated Validated Isotype IgG