![[KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)](/upload/resize_cache/iblock/5bb/340_340_140cd750bba9870f18aada2478b24840a/vjomyj03mtfwykwy6hhuqcyj750nuq1k.jpg "[KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)
- Описание
- Характеристики
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Overview
Product name [KO Validated] HP1 gamma/CBX3 Rabbit pAb Catalog No. A2248 Host species Rabbit Purification method Affinity purification Isotype IgG Background
At the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. This protein binds histone H3 tails methylated at Lys-9 sites. This protein is also recruited to sites of ultraviolet-induced DNA damage and double-strand breaks. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human HP1 gamma/HP1 gamma/CBX3 (NP_009207.2). Sequence MASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEAFLNSQKAGKEKDGTKRKSLSDSESDDSKSKKKRDAADKPRGFARGLDPERIIGATDSSGELMFLMKWKDSDEADLVLAKEANMKCPQIVIAFYEERLTWHSCPEDEAQ Gene ID Swiss Prot Synonyms HECH; HP1-GAMMA; HP1Hs-gamma Calculated MW 21kDa Observed MW 23kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples HeLa, MCF-7, 293T, A-549, NCI-H460, Mouse brain, Mouse lung, Mouse spleen Cellular location Nucleus Customer validation WB(Mus musculus)
IHC(Mus musculus)
Western blot analysis of extracts of various cell lines, using HP1 gamma/HP1 gamma/CBX3 antibody (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot analysis of extracts from normal (control) and HP1 gamma/HP1 gamma/CBX3 knockout (KO) 293T cells, using HP1 gamma/HP1 gamma/CBX3 antibody (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of C6 cells using HP1 gamma/HP1 gamma/CBX3 antibody (A2248) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using HP1 gamma/HP1 gamma/CBX3 antibody (A2248) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg HP1 gamma/CBX3 antibody (A2248). Western blot was performed from the immunoprecipitate using HP1 gamma/CBX3 antibody (A2248) at a dilution of 1:1000.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human HP1 gamma/HP1 gamma/CBX3 (NP_009207.2). KO Validated Validated Isotype IgG