![[KO Validated] LKB1 Rabbit mAb (A22636)](/upload/resize_cache/iblock/7fc/340_340_140cd750bba9870f18aada2478b24840a/1bfbi70d3awu4n8lnc506dgjr2gcusvy.jpg "[KO Validated] LKB1 Rabbit mAb (A22636)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] LKB1 Rabbit mAb (A22636)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] LKB1 Rabbit mAb Catalog No. A22636 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC58343 Background
The protein encoded by this gene is a serine/threonine kinase that regulates cell polarity and energy metabolism and functions as a tumor suppressor. Mutations in this gene have been associated with the autosomal dominant Peutz-Jeghers syndrome, as well as with skin, pancreatic, and testicular cancers.Immunogen information
Immunogen Recombinant protein of human LKB1. Sequence Email for sequence Gene ID Swiss Prot Synonyms PJS; LKB1; hLKB1 Calculated MW 49kDa Observed MW 49-60kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IP Recommended dilution - WB 1:2000 - 1:8000
- IHC-P 1:500 - 1:1000
- IP 1:2000 - 1:8000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Positive samples 293T, MCF7, L929, mouse testis, PC-12 Cellular location Cytoplasm, Membrane, Mitochondrion, Nucleus 
Western blot analysis of extracts from wild type(WT) and LKB1 knockout (KO) 293T cells, using LKB1 antibody (A22636) at 1:7000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry analysis of paraffin-embedded human kidney using [KO Validated] LKB1 Rabbit mAb (A22636) at dilution of 1:900 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human testis using [KO Validated] LKB1 Rabbit mAb (A22636) at dilution of 1:900 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using [KO Validated] LKB1 Rabbit mAb (A22636) at dilution of 1:900 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat testis using [KO Validated] LKB1 Rabbit mAb (A22636) at dilution of 1:900 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg LKB1 antibody (A22636). Western blot was performed from the immunoprecipitate using LKB1 antibody (A22636) at a dilution of 1:7000.
-
Key applications Western blotting, Immunohistochemistry, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human LKB1. KO Validated Validated Isotype IgG