![[KO Validated] NF-kB p65/RelA Rabbit pAb (A22683)](/upload/resize_cache/iblock/b61/340_340_140cd750bba9870f18aada2478b24840a/p82gharomu5xaqkobyz03wc1b0npmdcb.jpg "[KO Validated] NF-kB p65/RelA Rabbit pAb (A22683)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] NF-kB p65/RelA Rabbit pAb (A22683)
- Описание
- Характеристики
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Overview
Product name [KO Validated] NF-kB p65/RelA Rabbit pAb Catalog No. A22683 Host species Rabbit Purification method Affinity purification Isotype IgG Background
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant protein of human NF-kB p65/RelA. Sequence Email for sequence Gene ID Swiss Prot Synonyms p65; CMCU; NFKB3; AIF3BL3 Calculated MW 60kDa Observed MW 65kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC Recommended dilution - WB 1:2000 - 1:10000
- IHC-P 1:100 - 1:500
- IF/ICC 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Positive samples HeLa, COS-7, NIH/3T3, Mouse lung, PC-12 Cellular location cytoplasm, cytosol, glutamatergic synapse, nucleoplasm, nucleus 
Western blot analysis of various lysates, using NF-kB p65/RelA Rabbit pAb (A22683) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunohistochemistry of paraffin-embedded human liver using [KO Validated] NF-kB p65/RelA Rabbit pAb (A22683) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using [KO Validated] NF-kB p65/RelA Rabbit pAb (A22683) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using [KO Validated] NF-kB p65/RelA Rabbit pAb (A22683) at dilution of 1:300 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of HepG2 cells using [KO Validated] NF-kB p65/RelA Rabbit pAb (A22683) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] NF-kB p65/RelA Rabbit pAb (A22683) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant protein of human NF-kB p65/RelA. KO Validated Validated Isotype IgG