![[KO Validated] NEUROG3 Rabbit pAb (A23064)](/upload/resize_cache/iblock/364/340_340_140cd750bba9870f18aada2478b24840a/p158ngmhdnt0snhvlnnnn5eui7o8n4hu.jpg "[KO Validated] NEUROG3 Rabbit pAb (A23064)")
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Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] NEUROG3 Rabbit pAb (A23064)
- Описание
- Характеристики
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Overview
Product name [KO Validated] NEUROG3 Rabbit pAb Catalog No. A23064 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a basic helix-loop-helix (bHLH) transcription factor involved in neurogenesis. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of congenital malabsorptive diarrhea 4 (DIAR4).Immunogen information
Immunogen Recombinant protein of human NEUROG3. Sequence MTPQPSGAPTVQVTRETERSFPRASEDEVTCPTSAPPSPTRTRGNCAEAEEGGCRGAPRKLRARRGGRSRPKSELALSKQRRSRRKKANDRERNRMHNLNSALDALRGVLPTFPDDAKLTKIETLRFAHNYIWALTQTLRIADHSLYALEPPAPHCGELGSPGGSPGDWGSLYSPVSQAGSLSPAASLEERPGLLGATFSACLSPGSLAFSDFL Gene ID Swiss Prot Synonyms ngn3; Atoh5; NGN-3; Math4B; bHLHa7 Calculated MW 23kDa Observed MW 27kDa Applications
Reactivity Human Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Nucleus 
Western blot analysis of extracts from wild type(WT) and NEUROG3 knockout (KO) 293T(KO) cells, using NEUROG3 antibody (A23064) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant protein of human NEUROG3. KO Validated Validated Isotype IgG