![[KO Validated] CETN2 Rabbit pAb (A5397)](/upload/resize_cache/iblock/086/340_340_140cd750bba9870f18aada2478b24840a/8p6dbsf2bi7wg8qneuwmhb5wzec3ow88.jpg "[KO Validated] CETN2 Rabbit pAb (A5397)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] CETN2 Rabbit pAb (A5397)
- Описание
- Характеристики
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Overview
Product name [KO Validated] CETN2 Rabbit pAb Catalog No. A5397 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Caltractin belongs to a family of calcium-binding proteins and is a structural component of the centrosome. The high level of conservation from algae to humans and its association with the centrosome suggested that caltractin plays a fundamental role in the structure and function of the microtubule-organizing center, possibly required for the proper duplication and segregation of the centrosome.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN2 (NP_004335.1). Sequence MASNFKKANMASSSQRKRMSPKPELTEEQKQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFGDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVSEQEFLRIMKKTSLY Gene ID Swiss Prot Synonyms CALT; CEN2 Calculated MW 20kDa Observed MW 20kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, 293T, Mouse brain, Rat brain Cellular location Cytoplasm, Nucleus, centriole, centrosome, cytoskeleton, microtubule organizing center Customer validation WB(Homo sapiens)
IF(Homo sapiens)
Western blot analysis of extracts of various cell lines, using CETN2 antibody (A5397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of extracts from normal (control) and CETN2 knockout (KO) HeLa cells, using CETN2 antibody (A5397) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN2 (NP_004335.1). KO Validated Validated Isotype IgG