![[KO Validated] NSD3/WHSC1L1 Rabbit pAb (A5577)](/upload/resize_cache/iblock/3e7/340_340_140cd750bba9870f18aada2478b24840a/b09xik59e85610vj5htua3ske8b1xwwo.jpg "[KO Validated] NSD3/WHSC1L1 Rabbit pAb (A5577)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] NSD3/WHSC1L1 Rabbit pAb (A5577)
- Описание
- Характеристики
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Overview
Product name [KO Validated] NSD3/WHSC1L1 Rabbit pAb Catalog No. A5577 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. This protein methylates histone H3 at lysine residues 4 and 27, which represses gene transcription. Two alternatively spliced variants have been described.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 400-600 of human NSD3/NSD3/WHSC1L1 (NP_060248.2). Sequence EEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRHSQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFALQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATSGSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIK Gene ID Swiss Prot Synonyms KMT3F; KMT3G; WHISTLE; WHSC1L1; pp14328 Calculated MW 162kDa Observed MW 180kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T Cellular location Chromosome, Nucleus 
Western blot analysis of extracts of various cell lines, using NSD3/NSD3/WHSC1L1 antibody (A5577) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
Western blot analysis of extracts from normal (control) and NSD3/WHSC1L1 knockout (KO) 293T cells, using NSD3/WHSC1L1 antibody (A5577) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 400-600 of human NSD3/NSD3/WHSC1L1 (NP_060248.2). KO Validated Validated Isotype IgG