![[KO Validated] APEH Rabbit pAb (A5893)](/upload/resize_cache/iblock/826/340_340_140cd750bba9870f18aada2478b24840a/ixxsyowvjvxpt4p8ssqzvh3gkwcpdy3r.jpg "[KO Validated] APEH Rabbit pAb (A5893)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] APEH Rabbit pAb (A5893)
- Описание
- Характеристики
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Overview
Product name [KO Validated] APEH Rabbit pAb Catalog No. A5893 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3). Sequence MERQVLLSEPEEAAALYRGLSRQPALSAACLGPEVTTQYGGQYRTVHTEWTQRDLERMENIRFCRQYLVFHDGDSVVFAGPAGNSVETRGELLSRESPSGTMKAVLRKAGGTGPGEEKQFLEVWEKNRKLKSFNLSALEKHGPVYEDDCFGCLSWSHSETHLLYVAEKKRPKAESFFQTKALDVSASDDEIARLKKPDQAIKGDQFVFYEDWGENMVSKSIPVLCVLDVESGNISVLEGVPENVSPGQAFWAPGDAGVVF Gene ID Swiss Prot Synonyms APH; OPH; AARE; ACPH; D3S48E; D3F15S2; DNF15S2 Calculated MW 81kDa Observed MW 81kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IP Recommended dilution - WB 1:500 - 1:1000
- IP 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunoprecipitation Positive samples 293T, Jurkat Cellular location Cytoplasm 
Western blot analysis of various lysates, using APEH antibody (A5893) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts from wild type(WT) and APEH knockout (KO) 293T(KO) cells, using APEH antibody (A5893) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunoprecipitation analysis of 100 μg extracts of SW480 cells using 3 μg APEH antibody (A5893). Western blot was performed from the immunoprecipitate using APEH antibody (A5893) at a dilution of 1:1000.
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Key applications Western blotting, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human APEH (NP_001631.3). KO Validated Validated Isotype IgG