![[KO Validated] ING5 Rabbit pAb (A7288)](/upload/resize_cache/iblock/177/340_340_140cd750bba9870f18aada2478b24840a/w9yqt3y3ql0dnj67sabkia1wkv211dsk.jpg "[KO Validated] ING5 Rabbit pAb (A7288)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] ING5 Rabbit pAb (A7288)
- Описание
- Характеристики
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Overview
Product name [KO Validated] ING5 Rabbit pAb Catalog No. A7288 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a tumor suppressor protein that inhibits cell growth and induces apoptosis. This protein contains a PHD-type zinc finger. It interacts with tumor suppressor p53 and p300, a component of the histone acetyl transferase complex, suggesting a role in transcriptional regulation. Alternative splicing and the use of multiple promoters and 3' terminal exons results in multiple transcript variants.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ING5 (NP_115705.2). Sequence MATAMYLEHYLDSIENLPCELQRNFQLMRELDQRTEDKKAEIDILAAEYISTVKTLSPDQRVERLQKIQNAYSKCKEYSDDKVQLAMQTYEMVDKHIRRLDADLARFEADLKDKMEGSDFESSGGRGLKKGRGQKEKRGSRGRGRRTSEEDTPKKKKHKGGSEFTDTILSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIGCDNPDCPIEWFHFACVDLT Gene ID Swiss Prot Synonyms p28ING5 Calculated MW 28kDa Observed MW 30kDa Applications
Reactivity Human Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:1000
- IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples 293T Cellular location Nucleus Customer validation WB(Mus musculus)
Western blot analysis of extracts from normal (control) and ING5 knockout (KO) 293T cells, using ING5 antibody (A7288) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence analysis of MCF-7 cells using ING5 antibody (A7288). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ING5 (NP_115705.2). KO Validated Validated Isotype IgG