![[KO Validated] AMPKα2 Rabbit pAb (A7339)](/upload/resize_cache/iblock/e21/340_340_140cd750bba9870f18aada2478b24840a/4gmb3stnhtog1kirdpzb96hsve7n8236.jpg "[KO Validated] AMPKα2 Rabbit pAb (A7339)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] AMPKα2 Rabbit pAb (A7339)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] AMPKα2 Rabbit pAb Catalog No. A7339 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 343-552 of human AMPKα2 (NP_006243.2). Sequence ASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR Gene ID Swiss Prot Synonyms AMPK; AMPK2; PRKAA; AMPKa2 Calculated MW 62kDa Observed MW 62kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IF/ICC Recommended dilution - WB 1:100 - 1:500
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, 293T, HCT116, Mouse heart, Rat liver Cellular location Cytoplasm, Nucleus Customer validation WB(Homo sapiens, Gallus gallus, Mus musculus)
Western blot analysis of extracts of Rat liver, using AMPKα2 antibody (A7339) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
Western blot analysis of extracts of various cell lines, using AMPKα2 antibody (A7339) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from normal (control) and AMPKα2 Rabbit pAb knockout (KO) HCT116 cells, using AMPKα2 Rabbit pAb antibody (A7339) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s. -
Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 343-552 of human AMPKα2 (NP_006243.2). KO Validated Validated Isotype IgG