![[KO Validated] MST1/STK4 Rabbit pAb (A8043)](/upload/resize_cache/iblock/03b/340_340_140cd750bba9870f18aada2478b24840a/zlkmwu2g4xrym62cqg7olh1byskyxlbk.jpg "[KO Validated] MST1/STK4 Rabbit pAb (A8043)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] MST1/STK4 Rabbit pAb (A8043)
- Описание
- Характеристики
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Overview
Product name [KO Validated] MST1/STK4 Rabbit pAb Catalog No. A8043 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 303-487 of human MST1/STK4 (NP_006273.1). Sequence KRQESQQREVDQDDEENSEEDEMDSGTMVRAVGDEMGTVRVASTMTDGANTMIEHDDTLPSQLGTMVINAEDEEEEGTMKRRDETMQPAKPSFLEYFEQKEKENQINSFGKSVPGPLKNSSDWKIPQDGDYEFLKSWTVEDLQKRLLALDPMMEQEIEEIRQKYQSKRQPILDAIEAKKRRQQNF Gene ID Swiss Prot Synonyms KRS2; MST1; YSK3 Calculated MW 56kDa Observed MW 56kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples 293T, U-87MG, HepG2, HeLa, MCF-7, Mouse brain, Mouse lung, Mouse spleen, Rat liver Cellular location Cytoplasm, Nucleus Customer validation IF(Homo sapiens)
WB(Mus musculus)
Western blot analysis of extracts of various cells, using MST1/STK4 antibody (A8043) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.
Western blot analysis of extracts from normal (control) and MST1/STK4 knockout (KO) 293T cells, using MST1/STK4 antibody (A8043) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunoprecipitation analysis of 100 μg extracts of HepG2 cells using 3 μg MST1/STK4 antibody (A8043). Western blot was performed from the immunoprecipitate using MST1/STK4 antibody (A8043) at a dilution of 1:1000.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 303-487 of human MST1/STK4 (NP_006273.1). KO Validated Validated Isotype IgG