![[KO Validated] HDAC1 Rabbit pAb (A0238)](/upload/resize_cache/iblock/d0c/340_340_140cd750bba9870f18aada2478b24840a/z3t31qfrjl4ndrdcj0ablpsdg7hk0tue.jpg "[KO Validated] HDAC1 Rabbit pAb (A0238)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] HDAC1 Rabbit pAb (A0238)
- Описание
- Характеристики
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Overview
Product name [KO Validated] HDAC1 Rabbit pAb Catalog No. A0238 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2). Sequence SGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA Gene ID Swiss Prot Synonyms HD1; RPD3; KDAC1; GON-10; RPD3L1 Calculated MW 55kDa Observed MW 62kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:100
- IP 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples 293T, HeLa, HepG2, MCF7, Mouse thymus, Mouse testis, Rat thymus, Rat testis Cellular location Nucleus Customer validation IF(Human acute leukemia cells)
WB(Human acute leukemia cells, HL60,Hut102, 293T, Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa)
IP(Human acute leukemia cells)
IP(Rattus norvegicus)
ChIP(Rattus norvegicus)
Western blot analysis of extracts of various cell lines, using HDAC1 antibody (A0238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (A0238) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat spleen using [KO Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using [KO Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon using [KO Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A549 cells using HDAC1 antibody (A0238). Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of L929 cells using HDAC1 Polyclonal Antibody (A0238) at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg HDAC1 antibody (A0238). Western blot was performed from the immunoprecipitate using HDAC1 antibody (A0238) at a dilution of 1:1000.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2). KO Validated Validated Isotype IgG