![[KO Validated] DJ-1/PARK7 Rabbit pAb (A0987)](/upload/resize_cache/iblock/02c/340_340_140cd750bba9870f18aada2478b24840a/weyw17fup5886esy44rp61yr3jueodij.jpg "[KO Validated] DJ-1/PARK7 Rabbit pAb (A0987)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] DJ-1/PARK7 Rabbit pAb (A0987)
- Описание
- Характеристики
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Overview
Product name [KO Validated] DJ-1/PARK7 Rabbit pAb Catalog No. A0987 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human DJ-1/PARK7 (NP_001116849.1). Sequence MASKRALVILAKGAEEMETVIPVDVMRRAGIKVTVAGLAGKDPVQCSRDVVICPDASLEDAKKEGPYDVVVLPGGNLGAQNLSESAAVKEILKEQENRKGLIAAICAGPTALLAHEIGFGSKVTTHPLAKDKMMNGGHYTYSENRVEKDGLILTSRGPGTSFEFALAIVEALNGKEVAAQVKAPLVLKD Gene ID Swiss Prot Synonyms DJ1; DJ-1; GATD2; HEL-S-67p Calculated MW 20kDa Observed MW 20kDa Applications
Reactivity Human, Mouse Tested applications WB IF/ICC Recommended dilution - WB 1:500 - 1:2000
- IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunofluorescence Positive samples HeLa, NIH/3T3, Jurkat Cellular location Cell membrane, Cytoplasm, Lipid-anchor, Membrane raft, Mitochondrion, Nucleus Customer validation IF(Homo sapiens)
Western blot analysis of extracts of various cell lines, using DJ-1/PARK7 antibody (A0987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and DJ-1/PARK7 knockout (KO) 293T cells, using DJ-1/PARK7 antibody (A0987) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunofluorescence analysis of HeLa cells using DJ-1/PARK7 antibody (A0987). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunofluorescence Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-189 of human DJ-1/PARK7 (NP_001116849.1). KO Validated Validated Isotype IgG