![[KO Validated] LRRC59 Rabbit pAb (A10022)](/upload/resize_cache/iblock/0ea/340_340_140cd750bba9870f18aada2478b24840a/j669lt1uh62bm8ds2fqaormw9dlz6wp8.jpg "[KO Validated] LRRC59 Rabbit pAb (A10022)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] LRRC59 Rabbit pAb (A10022)
- Описание
- Характеристики
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Overview
Product name [KO Validated] LRRC59 Rabbit pAb Catalog No. A10022 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Enables RNA binding activity and cadherin binding activity. Predicted to be involved in positive regulation of Ras protein signal transduction and signal transduction. Located in endoplasmic reticulum and mitochondrial nucleoid.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human LRRC59 (NP_060979.2). Sequence MTKAGSKGGNLRDKLDGNELDLSLSDLNEVPVKELAALPKATILDLSCNKLTTLPSDFCGLTHLVKLDLSKNKLQQLPADFGRLVNLQHLDLLNNKLVTLPVSFAQLKNLKWLDLKDNPLDPVLAKVAGDCLDEKQCKQCANKVLQHMKAVQADQERERQRRLEVEREAEKKREAKQRAKEAQERELRKREKAEEKERRRKEYDALKAAKREQEKKPKKEANQAPKSKSGSRPRKPPPRKHTRS Gene ID Swiss Prot Synonyms p34; PRO1855 Calculated MW 35kDa Observed MW 35kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples 293T Cellular location Endoplasmic reticulum membrane, Microsome membrane, Nucleus envelope, Single-pass type II membrane protein 
Western blot analysis of extracts from wild type(WT) and LRRC59 knockout (KO) 293T(KO) cells, using LRRC59 antibody (A10022) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded rat cerebellum using LRRC59 Rabbit pAb (A10022) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human brain using LRRC59 Rabbit pAb (A10022) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse stomach using LRRC59 Rabbit pAb (A10022) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-244 of human LRRC59 (NP_060979.2). KO Validated Validated Isotype IgG