![[KO Validated] IFNGR1 Rabbit mAb (A11653)](/upload/resize_cache/iblock/20f/340_340_140cd750bba9870f18aada2478b24840a/0k2jeop4auc90gfj6zyx0u0kpt07ttp8.jpg "[KO Validated] IFNGR1 Rabbit mAb (A11653)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] IFNGR1 Rabbit mAb (A11653)
- Описание
- Характеристики
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Overview
Product name [KO Validated] IFNGR1 Rabbit mAb Catalog No. A11653 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC50421 Background
This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 270-489 of human IFNGR1 (NP_000407.1). Sequence IKKINPLKEKSIILPKSLISVVRSATLETKPESKYVSLITSYQPFSLEKEVVCEEPLSPATVPGMHTEDNPGKVEHTEELSSITEVVTTEENIPDVVPGSHLTPIERESSSPLSSNQSEPGSIALNSYHSRNCSESDHSRNGFDTDSSCLESHSSLSDSEFPPNNKGEIKTEGQELITVIKAPTSFGYDKPHVLVDLLVDDSGKESLIGYRPTEDSKEFS Gene ID Swiss Prot Synonyms CD119; IFNGR; IMD27A; IMD27B Calculated MW 54kDa Observed MW 45-90kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Positive samples 293T, BxPC-3, Mouse stomach, Mouse thymus, Rat lung Cellular location Membrane, Single-pass type I membrane protein 
Western blot analysis of various lysates, using IFNGR1 antibody (A11653) at1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
Western blot analysis of extracts from wild type(WT) and IFNGR1 knockout (KO) 293T cells, using IFNGR1 antibody (A11653) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Enhanced Kit (RM00021).
Exposure time: 180s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 270-489 of human IFNGR1 (NP_000407.1). KO Validated Validated Isotype IgG