![[KO Validated] LAMP2 Rabbit pAb (A14017)](/upload/resize_cache/iblock/76c/340_340_140cd750bba9870f18aada2478b24840a/43p80tfmmo1hbit79cctggy06zmluv12.jpg "[KO Validated] LAMP2 Rabbit pAb (A14017)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] LAMP2 Rabbit pAb (A14017)
- Описание
- Характеристики
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Overview
Product name [KO Validated] LAMP2 Rabbit pAb Catalog No. A14017 Host species Rabbit Purification method Affinity purification Isotype IgG Background
The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Alternative splicing of this gene results in multiple transcript variants encoding distinct proteins.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 59-263 of human LAMP2 (NP_002285.1). Sequence KTYKTVTISDHGTVTYNGSICGDDQNGPKIAVQFGPGFSWIANFTKAASTYSIDSVSFSYNTGDNTTFPDAEDKGILTVDELLAIRIPLNDLFRCNSLSTLEKNDVVQHYWDVLVQAFVQNGTVSTNEFLCDKDKTSTVAPTIHTTVPSPTTTPTPKEKPEAGTYSVNNGNDTCLLATMGLQLNITQDKVASVININPNTTHSTG Gene ID Swiss Prot Synonyms DND; LAMPB; CD107b; LAMP-2; LGP-96; LGP110 Calculated MW 45kDa Observed MW 100-130kDa Applications
Reactivity Human, Mouse Tested applications WB IHC-P IP Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunoprecipitation Positive samples HeLa, A-549, HepG2, Mouse liver Cellular location Cell membrane, Endosome membrane, Lysosome membrane, Single-pass type I membrane protein Customer validation IHC(Homo sapiens)
WB(Mus musculus)
Western blot analysis of extracts of various cell lines, using LAMP2 antibody (A14017) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Western blot analysis of extracts from normal (control) and LAMP2 knockout (KO) HeLa cells, using LAMP2 antibody (A14017) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded human liver using LAMP2 Rabbit pAb (A14017) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 59-263 of human LAMP2 (NP_002285.1). KO Validated Validated Isotype IgG