![[KO Validated] CTIP1/BCL11A Rabbit mAb (A9231)](/upload/resize_cache/iblock/5c8/340_340_140cd750bba9870f18aada2478b24840a/zxrwan42k3e81ihgkw8rffkrk16rg6vo.jpg "[KO Validated] CTIP1/BCL11A Rabbit mAb (A9231)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 200 μL
- 50 μL
[KO Validated] CTIP1/BCL11A Rabbit mAb (A9231)
- Описание
- Характеристики
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Overview
Product name [KO Validated] CTIP1/BCL11A Rabbit mAb Catalog No. A9231 Host species Rabbit Purification method Affinity purification Isotype IgG CloneNo. ARC1486 Background
This gene encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 401-589 of human CTIP1/BCL11A (Q9H165). Sequence GEKPYKCNLCDHACTQASKLKRHMKTHMHKSSPMTVKSDDGLSTASSPEPGTSDLVGSASSALKSVVAKFKSENDPNLIPENGDEEEEEDDEEEEEEEEEEEEELTESERVDYGFGLSLEAARHHENSSRGAVVGVGDESRALPDVMQGMVLSSMQHFSEAFHQVLGEKHKRGHLAEAEGHRDTCDEDS Gene ID Swiss Prot Synonyms EVI9; CTIP1; DILOS; ZNF856; HBFQTL5; SMARCM1 Calculated MW 91kDa Observed MW 120kDa Applications
Reactivity Human Tested applications WB IHC-P Recommended dilution - WB 1:500 - 1:1000
- IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Positive samples 293T Cellular location Cytoplasm, Nucleus 
Western blot analysis of extracts from wild type(WT) and CTIP1/BCL11A knockout (KO) 293T(KO) cells, using CTIP1/BCL11A Rabbit mAb (A9231) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using CTIP1/BCL11A Rabbit mAb (A9231) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Key applications Western blotting, Immunohistochemistry Host species Кролик Antibody type Primary Antibodies Reactivity Человек Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 401-589 of human CTIP1/BCL11A (Q9H165). KO Validated Validated Isotype IgG