![[KO Validated] NDUFA7 Rabbit pAb (A8441)](/upload/resize_cache/iblock/3d8/340_340_140cd750bba9870f18aada2478b24840a/hyj491egzb9q2z5eu9ivkl57rz0gdepp.jpg "[KO Validated] NDUFA7 Rabbit pAb (A8441)")
быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
[KO Validated] NDUFA7 Rabbit pAb (A8441)
- Описание
- Характеристики
-
Overview
Product name [KO Validated] NDUFA7 Rabbit pAb Catalog No. A8441 Host species Rabbit Purification method Affinity purification Isotype IgG Background
This gene encodes a subunit of NADH:ubiquinone oxidoreductase (complex I), which is a multiprotein complex located in the inner mitochondrial membrane. Complex I functions in the transfer of electrons from NADH to the respiratory chain.Immunogen information
Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2). Sequence MASATRLIQRLRNWASGHDLQGKLQLRYQEISKRTQPPPKLPVGPSHKLSNNYYCTRDGRRESVPPSIIMSSQKALVSGKPAESSAVAATEKKAVTPAPPIKRWELSSDQPYL Gene ID Swiss Prot Synonyms B14.5a; CI-B14.5a Calculated MW 13kDa Observed MW 13kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.Key application Western blotting Positive samples A-549, HepG2, MCF-7, Mouse kidney, Mouse brain, Mouse liver, Mouse heart, Rat liver Cellular location Matrix side, Mitochondrion inner membrane, Peripheral membrane protein 
Western blot analysis of extracts of various cell lines, using NDUFA7 antibody (A8441) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
Western blot analysis of extracts from normal (control) and NDUFA7 knockout (KO) 293T cells, using NDUFA7 antibody (A8441) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-113 of human NDUFA7 (NP_004992.2). KO Validated Validated Isotype IgG