быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
Pan Mono-Methyl lysine Rabbit pAb (A18293)
- Описание
- Характеристики
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Overview
Product name Pan Mono-Methyl lysine Rabbit pAb Catalog No. A18293 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Methylation of lysine residues is a common regulatory post-translational modification (PTM) that results in the mono-, di-, or tri-methylation of lysine at ε-amine groups by protein lysine methyltransferases (PKMTs).Immunogen information
Immunogen A synthetic peptide corresponding to a sequence containing monomethylated K. Sequence Email for sequence Gene ID Swiss Prot Synonyms Calculated MW Observed MW 17-90kDa Applications
Reactivity Human, Mouse, Rat Tested applications WB Recommended dilution - WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Positive samples HeLa, MCF7, NIH/3T3, C6, Mouse liver, Rat liver Cellular location Customer validation WB(Mus musculus)
IP(Homo sapiens)
Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine Rabbit pAb (A18293) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine Rabbit pAb (A18293) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
Western blot analysis of extracts of various cell lines, using Pan Mono-Methyl lysine antibody (A18293) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s. -
Key applications Western blotting Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса Immunogen A synthetic peptide corresponding to a sequence containing monomethylated K. Isotype IgG