быстрая доставка ~4 недели
Размер
- 100 μL
- 20 μL
- 200 μL
- 50 μL
MonoMethyl-Histone H4-R3 Rabbit pAb (A3158)
- Описание
- Характеристики
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Overview
Product name MonoMethyl-Histone H4-R3 Rabbit pAb Catalog No. A3158 Host species Rabbit Purification method Affinity purification Isotype IgG Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.Immunogen information
Immunogen A synthetic monomethylated peptide around R3 of human Histone H4 (NP_003529.1). Sequence SGRGK Gene ID Swiss Prot Synonyms H4/I; H4C1; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4FI; H4-16; H4C11; H4C12; H4C13; H4C14; H4C15; H4C16; HIST1H4B Calculated MW 11kDa Observed MW 13kDa Applications
Reactivity Human, Mouse, Rat, Other (Wide Range) Tested applications WB IHC-P IF/ICC IP Recommended dilution - WB 1:500 - 1:2000
- IHC-P 1:50 - 1:200
- IF/ICC 1:50 - 1:200
- IP 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.Key application Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation Positive samples HeLa Cellular location Chromosome, Nucleus 
Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H4-R3 antibody (A3158).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry analysis of paraffin-embedded rat lung using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat brain using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human breast cancer using MonoMethyl-Histone H4-R3 Rabbit pAb (A3158) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of 293T cells using MonoMethyl-Histone H4-R3 antibody (A3158). Blue: DAPI for nuclear staining.
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Key applications Western blotting, Immunohistochemistry, Immunofluorescence, Immunoprecipitation Host species Кролик Antibody type Primary Antibodies Reactivity Человек, Мышь, Крыса, Другое (Широкий ассортимент) Immunogen A synthetic monomethylated peptide around R3 of human Histone H4 (NP_003529.1). Isotype IgG